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ChinaPeptides 3xflag peptide
3xflag Peptide, supplied by ChinaPeptides, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3xflag+peptide/3xflag+peptide/pm40653822-128-31-34
Average 90 stars, based on 1 article reviews
3xflag peptide - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Recombinant:

Article Title: A phosphorylation of RIPK3 kinase initiates an intracellular apoptotic pathway that promotes prostaglandin2α-induced corpus luteum regression
Article Snippet: DOI: https://doi.org/10.7554/eLife.67409 20 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Donkey anti-Mouse, Alexa Fluor 555 (Mouse polyclonal) Thermo Fisher Cat# A-31570; RRID:AB_2536180 IF (1:500) Antibody Donkey anti-Rabbit, Alexa Fluor 488 (Rabbit polyclonal) Thermo Fisher Cat# A-21206; RRID:AB_141708 IF (1:500) Antibody Donkey anti-Rabbit, Alexa Fluor 555 (Rabbit polyclonal) Thermo Fisher Cat# A-31572; RRID:AB_162543 IF (1:500) Antibody Anti-Flag M2 affinity gel Sigma-Aldrich A2220 Recombinant DNA reagent pWPI-HA-3xFlagRIPK3 This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(D160N) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(K50A) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(AAAA) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagmRIPK3 This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(S164D/T165E) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(S164E) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(T165E) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(S164A) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(T165A) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(S164A/T165A) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagmRIPK3(D161N) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagmRIPK3(S165D/T166E) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pLVX-Tight-HA-3xFlag-RIPK3 This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pLVX-Tight-HA-3xFlagRIPK3(D160N) This paper N/A Described in Materials and methods; available upon request Continued on next page Li et al. eLife 2021;10:e67409. .. DOI: https://doi.org/10.7554/eLife.67409 21 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Recombinant DNA reagent pLVX-Tight-HA-3xFlagRIPK3(K50A) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pLVX-Tight-HA-3xFlagRIPK3(S164A/T165A) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pX458-GFP-RIPK1 This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pX458-GFP-caspase-8 This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pX458-GFP-FADD This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pX458-GFP-cFLIP This paper N/A Described in Materials and methods; available upon request Peptide, recombinant protein 3xFlag peptide ChinaPeptides DYKDHDGDYKD HDIDYKDDDDK 1 mg/ml Software, algorithm ImageJ NIH N/A Software, algorithm Photoshop Adobe N/A Software, algorithm Lasergene DNASTAR N/A Software, algorithm GraphPad GraphPad Software N/A Software, algorithm Prism GraphPad Software N/A Software, algorithm Nikon A1-R Nikon https://www. nikoninstruments. com/ Products/Confocal-Microscopes/A1R-HD ..

Article Title: A phosphorylation of RIPK3 kinase initiates an intracellular apoptotic pathway that promotes prostaglandin 2α -induced corpus luteum regression
Article Snippet: Recombinant DNA reagent , pX458-GFP-cFLIP , This paper , N/A , Described in Materials and methods; available upon request. .. Peptide, recombinant protein , 3xFlag peptide , ChinaPeptides , DYKDHDGDYKDHDIDYKDDDDK , 1 mg/ml. .. Software, algorithm , ImageJ , NIH , N/A , .

Software:

Article Title: A phosphorylation of RIPK3 kinase initiates an intracellular apoptotic pathway that promotes prostaglandin2α-induced corpus luteum regression
Article Snippet: DOI: https://doi.org/10.7554/eLife.67409 20 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Donkey anti-Mouse, Alexa Fluor 555 (Mouse polyclonal) Thermo Fisher Cat# A-31570; RRID:AB_2536180 IF (1:500) Antibody Donkey anti-Rabbit, Alexa Fluor 488 (Rabbit polyclonal) Thermo Fisher Cat# A-21206; RRID:AB_141708 IF (1:500) Antibody Donkey anti-Rabbit, Alexa Fluor 555 (Rabbit polyclonal) Thermo Fisher Cat# A-31572; RRID:AB_162543 IF (1:500) Antibody Anti-Flag M2 affinity gel Sigma-Aldrich A2220 Recombinant DNA reagent pWPI-HA-3xFlagRIPK3 This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(D160N) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(K50A) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(AAAA) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagmRIPK3 This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(S164D/T165E) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(S164E) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(T165E) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(S164A) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(T165A) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagRIPK3(S164A/T165A) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagmRIPK3(D161N) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pWPI-HA-3xFlagmRIPK3(S165D/T166E) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pLVX-Tight-HA-3xFlag-RIPK3 This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pLVX-Tight-HA-3xFlagRIPK3(D160N) This paper N/A Described in Materials and methods; available upon request Continued on next page Li et al. eLife 2021;10:e67409. .. DOI: https://doi.org/10.7554/eLife.67409 21 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Recombinant DNA reagent pLVX-Tight-HA-3xFlagRIPK3(K50A) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pLVX-Tight-HA-3xFlagRIPK3(S164A/T165A) This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pX458-GFP-RIPK1 This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pX458-GFP-caspase-8 This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pX458-GFP-FADD This paper N/A Described in Materials and methods; available upon request Recombinant DNA reagent pX458-GFP-cFLIP This paper N/A Described in Materials and methods; available upon request Peptide, recombinant protein 3xFlag peptide ChinaPeptides DYKDHDGDYKD HDIDYKDDDDK 1 mg/ml Software, algorithm ImageJ NIH N/A Software, algorithm Photoshop Adobe N/A Software, algorithm Lasergene DNASTAR N/A Software, algorithm GraphPad GraphPad Software N/A Software, algorithm Prism GraphPad Software N/A Software, algorithm Nikon A1-R Nikon https://www. nikoninstruments. com/ Products/Confocal-Microscopes/A1R-HD ..

Incubation:

Article Title: Characterization of WAC interactions with R2TP and TTT chaperone complexes linking glucose and glutamine availability to mTORC1 activity.
Article Snippet: .. Unbound proteins were extensively washed with WAC buffer B2, and elution was performed by incubation for 30 min at 4 °C with WAC buffer B2 supplemented with 0.3 mg mL 1 3xFlag peptide (MDYKDHDGDYKDHDIDYKDDDDK, ChinaPeptides). ..

Article Title: Characterization of WAC interactions with R2TP and TTT chaperone complexes linking glucose and glutamine availability to mTORC1 activity.
Article Snippet: .. WAC protein was eluted by incubation for 30 min at 4 °C with WAC buffer B supplemented with 3xFlag peptide (MDYKDHDGDYKDHDIDYKDDDDK, QYAOBIO (ChinaPeptides), Shangai, China) (final concentration 0.3 mg mL 1). ..

Concentration Assay:

Article Title: Characterization of WAC interactions with R2TP and TTT chaperone complexes linking glucose and glutamine availability to mTORC1 activity.
Article Snippet: .. WAC protein was eluted by incubation for 30 min at 4 °C with WAC buffer B supplemented with 3xFlag peptide (MDYKDHDGDYKDHDIDYKDDDDK, QYAOBIO (ChinaPeptides), Shangai, China) (final concentration 0.3 mg mL 1). ..

other:




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